山莨菪碱对毒蕈所致大鼠急性肾损伤的保护作用

The protective effect of the anisodamine on acuterenal damage caused by mushroom poisoning

随机将１７４只大鼠分为Ⅰ、Ⅱ、Ⅲ组，每组５８只动物。Ⅰ组：用等渗盐水灌胃；Ⅱ组：用毒蕈匀浆液灌胃；Ⅲ组：毒蕈匀浆液灌胃同时腹腔注射山莨菪碱（６５４－２）注射液，观察肾脏生化和形态学改变。Ⅱ组灌胃后６ｈ肾皮质自由基有关指标出现明显变化，肾近曲和远曲小管出现超微结构病变，２４ｈ后出现光镜下病变，尿钠、钾排泄增加，尿渗透压降低。Ⅲ组灌胃后１２ｈ肾皮质匀浆自由基有关指标出现异常变化，肾近曲和远曲小管出现超微结构病变，晚于Ⅱ组出现；４８ｈ后出现光镜下病变，尿钠、尿钾排泄增加，尿渗透压降低。说明６５个２对毒蕈所致大鼠急性肾损伤有一定的保护作用。

n order to investigate the protective eftect ofthe anisodamine (654-2) on acute renal damagecaused by mushroom poisoning , 174 rats were divid-ed randomly into three groups. Every group con-tains 58 rats. GroupⅠ: oral administration of 50 mlnormal saline per kg body weight; Group Ⅱ : oraladministration of 50 g fresh Amamita phalloides perkg body weighy; Group Ⅲ : the equal Amamita phal-loides, added 10 mg 654-2 by i. p. simultaneouslyper kg body weight. We observed the changes oflipid peroxides (LPO) content . superoxide dismu-tase (SOD), Catalase (Cat) , Na￣+ -K￣+ ATPase activi-ties in renal cortical (RC) homogenate,as well asfunctional and morphological damage of the kidneysin rats administrated with the toxic rnushroom ho-mogenate by garage before treatment and 1,2, 12,24 , 48 hours after treatment. The RC LPO content(in Group Ⅲ ) was lower than Group Ⅱ at 6 hours.The RC SOD,Cat and Na￣+-K￣+ ATPase activities inGroup Ⅲ were higher than those in Group Ⅱ at 6 , 12or 24 hours. The renal functional and morphologicaldamage in Group Ⅲ were less than those in GroupⅡ before 24 hours. Our data suggested that aniso-damine had protective effect on acute renal damagecaused by mushroom poisoning ,and could decreasefree radicals reactions.