大鼠延髓腹外侧表面呼吸调节化学感受区的感受机制分析

On the Chemoreception Mechanism of the Ventrolateral Medullary Surface in Rats

用免疫组化（ＨＲＰ）、Ｈ＋表面透入、核团微量注射、微电泳及损毁等方法探讨了延髓腹侧表面中枢化学感受机制。结果表明它与其浅层核团：斜方体核、外周橄榄腹外侧核（ＬＶＰＯ）、斜方体后核、巨细胞旁外侧核和外侧网状核等有神经结构联系。表面Ｈ＋可能被上述核团的突起或胞体感受。非呼吸相关神经元（ＬＶＰＯ）与呼吸相关神经元（面神经后核，ＮＲＦ）同样可能参与中枢化学感受而调节呼吸活动。

This work is to investigate the mechanism of central chemoreception of the ventral medullary surface in respiratory regulation in rats. A series of methods including immunohistochemical(HRP),H+ permeation, microinjection, microiontophoresis,and destruction of related nuclei were used. Results showed that nucleus of trapezoid, lateroventral periolivary nucleus(LVPO), retrotrapezoid nucleus, lateral paragigantocellular nucleus and lateral reticular nucleus have the neural structural relations with the surface. Their processes or their soma might be receptive to the H+ applied to the ventral surface.It also demonstrated that both non-respiration related neuron(LVPO)and respiration related neuron(nucleus retrofacialis, NRF)might play roles in central chemoreception in respiratory regulation.