脑内微透析采样技术及其在神经科学中的应用

Intracerebral Microdislysis and Its Application in Neurosciences

作为一种新的在体（ｉｎｖｉｖｏ）化学采样技术，脑内微透析引起了神经科学家的关注。它与迅速发展起来的高灵敏度的微量化学分析技术相结合，实现了对体内细胞外环境中化学物质变化的动态监测，从而在神经科学领域获得应用。本文系统地介绍了这一新技术的原理和方法，并扼要地介绍了这一技术在神经科学中的应用及其取得的新进展。并结合本实验室的工作经验，对该技术存在的一些问题进行了讨论。

As a new technique for sampling the extracellular fluid in vivo,the microdialysis is recently attractive to neuroscientists.Coupled with high sensitivity techniques for chemical microanalysis,it has been successfully used for monitoring chemical changes in extracellular environment of intact brain,and applied to some fields of neuroscience studies.In this article,its principle and some methodological aspects were described systematically,and a brief overview of its applications in neurosciences and some new advances was also given. Besides, the scope and limitations of microdialysis was discussed in the light of our own experience.