电针刺激下即刻早期基因和前脑啡肽基因在中枢神经系统中的表达及定位研究

Expression and Localization of Immediate Early Genes and Preproenkephalin Gene in Central Nervous System Following Eletroacupuncture Stimulation

采用免疫组织化学、Ｎｏｒｔｈｅｒｎ杂交与原位杂交等方法，观察到２／１５Ｈ电针刺激可诱导大鼠中枢神经系统内即刻早期基因ｃ－ｆｏｓ、ｃ－ｊｕｎ的ｍＲＮＡ与免疫阳性物质以及前脑啡肽（ＰＥＮＫ）ｍＲＮＡ的生成。Ｎｏｒｔｈｅｒｎ杂交显示，电针在１～２ｈ内引起即刻早期基因ｃ－ｆｏｓｍＲＮＡ的表达，继之以ＰＥＮＫｍＲＮＡ的表达，在４８ｈ内方达顶点；形态学结果表明，电针诱导ｃ－ｆｏｓ、ｃ－ｊｕｎ与ＰＥＮＫｍＲＮＡ及其蛋白产物的生成在中枢的分布具有明显的一致性。提示Ｆｏｓ／Ｊｕｎ可能作为ＰＥＮＫ基因的转录调节因子。

Using the techniques of immunohistochemistry,Northern blotting and in situ hybridization,we have found that electroacupuncture(2/15Hz) accelerated the expression of mRNA and protein synthesis of immediate early genes c-fos,c-jun as well as proenkephalin gene in rat central nervous system(CNS).Northern hybridization revealed that EA stimulation induced c-fos gene expression within 1一2h,followed by PENK gene expression which peaked at 4 8h. Morphological study demonstrated that the sites of c-fos,c-jun and PENK mRNA expression and protein synthesis coincide with each other in the CNS.The results suggest that Fos/Jun may function as the transcription regulator of PENK gene.