人工合成寡肽对大鼠黄体细胞孕酮生成的调节及其可能作用机制

The Effects of Synthetic Oligopeptides on Progesterone Production in Corpus Luteum Cell of Rat and Their Mechanism of Action

本实验比较了合成寡肽抗孕酮生成作用，并进行了相应机制探讨。发现当ｐＨ７．３～７．５时，能较强抑制孕酮分泌的寡肽其结构有共同特点；活性寡肽可对ＰＬＣ信使传递系统产生抑制作用；也可能通过调节黄体细胞内钙离子浓度降低了ｈＣＧ致孕酮的生成作用；甘－丝－赖还升高黄体细胞中ＰＫＣ活性，而降低了ＰＫＡ。可见人工合成寡肽的抗孕酮作用分子机制十分复杂，有待于深入探讨。

The effects of a series of synthetic oligopeptides on progesterone production by rat CL cells were compared and their mechanism of actions was studied in vitro. The ones with inhibitory actions were characterized by carrying positive charge in the meduim of pH 7.3～ 7.5 and with intermolecular linkage.A preliminary survey of the effect of the active oligopeptides on signal systems showed:(1)GY and YG inhibited PLC system;(2)GY and GSK reduced hCG induced progesterone production in CL cells probably by regulating cellular Ca2+ concentration:(3) GSK decreased TPK activity and GYK increased it in hCG treated CL cell though both of them were inhibitory on progesterone production. GSK also stimulated PKC and supressed PKA activity in CL cells.The anti-progesterone effect of oligopeptides so far synthesized may influence either PKA or PKC or TPK systems.The mechanism of action at the molecular level is quite complicated.