国产河豚毒素（TTX）单克隆抗体8A5有效地拮抗TTX对钠流的抑制

DEPRESSION OF TTX-BLOCKING EFFECT ON Na￣+ CURRENT BY 8A5,AN ANTI-TTX MONOCLONAL ANTIBODY

利用克隆系ＮＧ１０８－１５细胞在膜片箝全细胞（ｗｈｏｌｅ－ｃｅｌｌｃｌａｍｐｒｅｃｏｒｄｉｎｇ）记录下，观察了国产ＴＴＸ单克隆抗体８Ａ５拮抗ＴＴＸ对跨膜钠电流的作用。结果表明，８Ａ５有效地抑制ＴＴＸ的作用：（１）在标准灌流液中加入１μｍｏｌ／ＬＴＴＸ，Ｎａ＋流立即被完全阻断，复加１μｍｏｌ／Ｌ８Ａ５于灌流液，可使Ｎａ＋流迅速恢复至对照水平；（２）若先以含１μｍｏｌ／Ｌ８Ａ５的溶液灌流细胞，再更置为含１μｍｏｌ／ＬＴＴＸ的标准液，Ｎａ＋流只有部分被抑制。

Anti-TTX effect of 8A5 on Na+ current was observed by whole-cell voltageclamp technique in NG108-15 cells. The results show that 8A5 inhibits TTX action efficiently: (1) When 1 μmol/L TTX is added to the normal perfusion solution ⅠNa disappears immediately, and can be restored to the control level with further addition of8A5. (2) When the cell is pre-incubated with 1 μmol/L 8A5, 1 μmol/L TTX can only cause a partial inhibition of ⅠNa.