摘要
应用核团微量注射、放射免疫测定(RIA)和高压液相(HPLC)观察了刺激视上核(SON)对蓝斑(LC)灌流液中催产素(OT)、精氨酸加压素(AVP)、去甲肾上腺素(NE)和5-羟色胺(5-HT)的含量的变化以及蓝斑注射OT和AVP的拮抗剂对痛阈(PT)的影响。结果表明:刺激SON后30到90min,LC灌流液中OT的含量,30minAVP的含量,60min5-HT的含量明显增加,而NE的含量在30和60min时明显降低。注射AVP的V1拮抗剂到LC对注射L-谷氨酸到SON引起的镇痛作用无影响,V2拮抗剂可部分抑制和OT拮抗剂可翻转刺激SON引起的镇痛作用。以上结果提示刺激SON引起的镇痛作用可能是由于SON释放的OT通过作用于LC内的OT和V2受体使LC内的5-HT增加和NE降低而产生的。
The effects of stimulation of supraoptic nucleus (SON) on the changes of oxytocin (OT), arginine vasopressin (AVP), norepinephrine (NE) and serotonin (5-HT) in the perfusate of locus coeruleus (LC) and changes of pain threshild (PT) were studied by methods of microinjection, radioimmunoassay (RIA) and high pressure liquid chromatography (HPLC) technique. The results showed that the OT contentS at 30, 60 and 90 min after stimulation, that of AVP at 30 min and 5-HT at 60 min were increased significantly, while the NE contents at 30 and 60 min were decreased markedly, Injection of V1 antagonist into LC did not affect the analgesic effect caused by administration of L-glutamic acid (L-Glu) into SON, which, however, could be partially inhibited by V2 antagonist or even reversed by OT antagonist. The above results suggested that the analgesic effect due to stimulation of SON is caused by increase of 5-HT and decreases of NE in the LC though the action of OT released by SON on OT and V2 receptor in LC.
出处
《生理学报》
CAS
CSCD
北大核心
1995年第4期320-326,共7页
Acta Physiologica Sinica
基金
国家自然科学基金
关键词
蓝斑
视上核
镇痛
locus coeruleus
supraoptic nucleus
oxytocin
serotonin
norepinephrine
analgesia
rat