脑室注射白细胞介素1受体拮抗剂对抗束缚应激免疫抑制因子的产生

INTRACEREBROVENTRICULAR INJECTION OF INTERLEUKIN 1 RECEPTOR ANTAGONIST ANTAGONISES ON THE GENERATION OF IMMUNO-SUPPRESSIVE FACTOR IN RESTRAINT MICE

我们以前的工作发现束缚应激小鼠血清里存在一种能抑制淋巴细胞转化的蛋白。本工作研究了脑内白细胞介素１（ＩＬ－１）对这种血清蛋白产生的作用。脑室注射白细胞介素１受体拮抗剂（ＩＬ－１Ｒａ）能抑制这种血清蛋白的产生，并呈量效关系。注射５．０μｇＩＬ－１Ｒａ时，几乎完全对抗此蛋白的产生。脑室注射１ｐｇＩＬ－１β则对此蛋白的产生有增强作用；腹腔注射ＩＬ－１β或ＩＬ－１Ｒａ，均无影响。以上结果表明脑内ＩＬ－１在束缚应激血清抑制蛋白的产生过程中起有很重要的作用。脑室注射ＩＬ－１β的剂量小至０．０６ｆｍｏｌ对血清抑制蛋白的产生有增强作用，表明脑内ＩＬ－１可能是中枢神经系统与免疫系统之间的一种重要调节物。

Our previous work showed that a lymphocyte proliferation suppressing factor could be found in the serum of restraint mice. In the present work, it was found that intracerebroventricular (icv) injection of IL-1 receptor antagonist (IL-1Ra) was found capable of suppressing the production of such a serum protein under restraint stress. Nearly complete suppression could be achieved by 5. 0μg IL-1Ra. Intracerebroventricular injection of IL-1β(1 pg), however, increased the generation of the supressive protein. Neither intraperitoneal (ip) injection of IL-1Ra or IL-1β had any effect on the generation of the protein. The fact that icv. injection of a very small dose of IL-1β (0. 06 fmol) was effective on the generation of the supressive protein led us to suggest that IL-1 in brain might act as an important mediator between CNS and the immune system.