离心薄层层析法分离半合成头孢菌素衍生物的研究

STUDY OF PREPARATIVE SEPARATION OF CEPHALOSPORINS BY CENTRIFUGAL TLC

本文用离心薄层层析法对半合成头孢菌素类化合物进行了制备量分离的研究。采用此法有效地分离了Ⅱ、Ⅲ两个系列共38个半合成头孢菌素衍生物,深入探讨了吸附层、洗脱剂、分离样品限量、分离时间等分离条件。结果表明:离心薄层层析法适用于半合成头孢菌素的分离,仪器简单,操作方便,经济省时,具有满意的分离效果。

The synthetic products of cephalosporin compounds are isolated by centrifugal TLC(Chromatotron) in preparative quantity. This separation techni-qus is satisfactorily applied to the purification of two series of, thirty-eight, ce-phalospdrin compounds.And the operating conditions including preparation and reuse of coating layer,selection of the solvent, limit of separation quantity, separation time etc.are studied. Centrifugal TLC is a simple and very rapid technique for the preparative separation or purification of cephalosporin compounds and proves to have the advantage of lower cost, less time and better availability.This method is much more suitable for the separation and purification of unstable substances like cephalospo-rins.