摘要
本文报道氯霉素产生菌委内瑞拉链霉菌A-186株与林可霉素产生菌林可链霉菌林可变种78-11株种间原生质体融合的结果。首先,研究了委内瑞拉链霉菌A-186的原生质体制备和再生条件,再生率可达86%。然后,采用氯霉素产生菌S.venezuelae A-186的原生质体用紫外线灭活后,与具有耐药标记的S.lincolnensis var.lincolnensis78-11原生质体融合的方法,种间融合频率达到3.2×10^(-5) 。并筛选到一株遗传稳定的重组子,经初步鉴定它能同时产生两亲株所产的两种抗生素。
The protoplast fusion between Streptomyces lincolnensis 78-11, a lin-comycin producer (CA^3, LM^2) and Streptomyces venezuelae A-186, a chloramphenicol producer (CA^3,LM^2) were studied. When the optimal conditions were used regeneration frequency of protoplast of Streptomyces venezuelae A-186 was about 86%.
When U. W. inactivated protoplast of S.venezuelae fused with untreated protoplast of 5. lincolnensis the recoinbinants incresed the frequency to 3.17x10^(-5).
Some genetically stable recombinants were screened.One of the recombinants produces both chloramphenicol and lincomycin. This result is important for studying genetic manipulation and metablic regulation in Streptomyces.
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
1989年第3期192-196,共5页
Chinese Journal of Antibiotics
关键词
林可链霉菌
原生质体融合
重组子
Str. lincolnensis
Str. venezulaei
Protoplast
Recombinant