淀粉凝胶与植物组织培养——Ⅱ.玉米淀粉凝胶培养基对苹果砧木组培生根诱导的同步化作用

Starch Gel and Tissue Culture:II. Synchronized Effects of Corn Starch Gel Medium on Rooting Induction of Malus Zumi and M26

珠美海棠和M26苹果矮化砧木分别在琼脂培养基和玉米淀粉(corn starch)凝胶培养基上进行生根培养,结果玉米淀粉培养基不但提高了两种植物的生根率,而且对生根诱导具有同步化效应。珠美海棠生根苗游离氨基酸含量的高效液相色谱(HPLC)分析表明,琼脂生根培养基上,吲哚丁酸(IBA)诱导了天冬氨酸、谷氨酸、组氨酸、苏氨酸、色、蛋氨酸和谷氨酰胺、丙、酪氨酸的降低,但却提高了精氨酸、缬氨酸、苯丙氨酸、亮氨酸和鸟氨酸的含量。当玉米淀粉替代琼脂后,玉米淀粉培养基不但进一步促进了上述氨基酸含量的降低,而且抑制了精氨酸、缬氨酸、苯丙氨酸和亮氨酸等的升高。

Malus zumi and M26 dwarfing stocks for apple trees were cultured in agar medium and corn starch gel medium (CSM) .respectively. The results showed that the CSM not only increased rooting rate of the two species ,but also had synchronized effests on rooting induction. For example ,the rooting rate in CSM reached 85-98%,23 -30% higher than that in agar medium,and the rooting differentiation was completed in about 2 to 3 days,which was also shorter than that in agar medium . HPLC analysis of free amino acids of M. zumi demonstrated that the CSM inhabited the increase of the content of Arg ,Val, Phe ,Leu ,ORn,and stimulated further reduction of other free amino acids in rooting culture.