人参培养细胞单细胞克隆的条件培养

Conditioning Culture of Single Cell Clone from Culture Cells of Panax ginseng

来自细胞悬浮培养物的条件培养基能显著地促进人参培养细胞的单细胞在较低植板密度培养时的克隆形成。每毫升含３×１０＾３个细胞时，条件培养的植板率是普通培养的４倍。细胞悬浮培养１２－１６天时所制备的条件培养基活性最大，在一定浓度范围内随条件培养基浓度增加，细胞克隆植板率随之增加。条件培养基具有一定的生理作用专一性。看护培养和条件增大的比较，表明前者在细胞密度较低时能更有效地促进细胞克隆的形成和生长。

Conditioned medium (CM) stemming from cell suspension culture could remarkably promote the formation of single cell clone from culture cells of Panax ginseng below a critical cell plating density. The plating efficiency of conditioning culture was 4 times as much as that of the normal plating culture if CM was added to the plating medium when cell plating density was 3×103/ml. The activity of CM would be the highest if it originated from cells cultured for 12-16 days. The plating efficiency was improved when the amount of CM in plating medium was increased within the ranges of proper concentrations. CM possessed species-specificity to some extent. The comparative study between nursing culture and conditioning culture indicated that the former could more effectively stimulate the formation and growth fo single cell clone at a lower cell plating density compared with the latter. CM stored for two weeks at 4℃ remained its stimulating activity Moreover, it was resistant to high temperature, weak acid or alkaline pH. but it was anstable when treated with strong acid and alkaline pH.