小麦胚性悬浮系与原生质体植株再生

Plant Regeneration of Wheat Protoplasts Prepared from Different Composition of Suspension Culture

普通小麦(Triticum aestivum)昌乐5号(冬性)胚性悬浮细胞系的组成成分对原生质体再生频率发生影响。此种悬浮系是由混合型愈伤组织建立起来的,建成的悬浮系中含有2—3mm的小愈伤组织和分散好的几十至上百个细胞的细胞团。分别用悬浮系中的小愈伤组织和细胞团分离原生质体进行培养。结果表明,由小愈伤组织来源的原生质体再生植株的频率显著高于由细胞团分离的原生质体的再生频率。培养基中不同成分对原生质体分裂的影响也作了研究。

Regenerable embryogenic cell suspensions initiated from immature embryo-derived 'mix-type' calli of winter wheat (Triticum aestivum L. ) Chang-le 5 served as sources of protoplast. Cell suspension consisted of small calli (2 -3mm in size) and cell clumps (0. 2 -mm in size). The regeneration frequency of the protoplasts derived from small calli of the suspension was remarkably higher than that of cell clumps. The effect of different composition in culture medium including macro elements, vitamins, glycine, glutamin and casein hydrolysate on protoplast division was also investigated.