生物学因子对紫苏悬浮培养细胞生长和花色素形成的影响

Effects of Biological Factors on Cell Growth and Anthocyanin Formation by Suspended Cultures of Perilla frutescens Cells

应用摇瓶培养研究了生物学因子,即:细胞聚集体大小、继代周期和接种量,对紫苏悬浮细胞生长和次生代谢物花色素产生的影响。结果表明:与未经筛选的或细胞聚集体小于250μm的细胞团块相比,大于250μm的细胞团块作为接种细胞时,培养所得的花色素含量较低。7—10无为合适的继代周期,在长时期的继代过程中,细胞生长良好、并且色素含量也高。实验还表明,每升接种50克湿细胞最适合于细胞增殖与色素积累。

Effects of biological factors,i, e. size of cell aggregates, subculture period, and inoculum density, on cell growth and production of anthocyanin pigment (a secondary metabolite)by suspended cultures of Perilla frutescens plant cells were investigated on a flask scale. It was found that anthocyanin content was lower in a culture inoculated with cells of sizes greater than 250μm,compared with cells with unscreened sizes or with their sizes smaller than 250μm. Subculture periods of 7 days and 10 days were suitable for the cell cultures because favorable growth and a high level of anthocyanin content were maintained during a long period of subculture. An inoculum density 50g/L(Wet cell)was the best for both the cell growth and the pigment accumulation in flask cultures of P. frutescens cells.