乙肝病毒S基因在家蚕细胞及蚕体内高效表达

High Expression of ,HBV S Gene in Bm Cell and Silkworm

把人乙型肝炎病毒（ａｄｒ）的表面抗原Ｓ基因插入到家蚕核型多角体病毒基因组中，构建了重组病毒ＢｍＮＰＶＳ。用重组病毒感染家蚕细胞，测得每毫升培养物（１×１０＾５细胞）ＨＢｓＡｇ表达量达３５．５ｕｇ；感 家蚕幼虫和蛹，经检测表明ＨＢｓＡｇ产量平均为每头蚕约７５０ｕｇ，每只蛹约为６９０ｕｇ。初步纯化的表达产物经Ｗｅｓｔｅｒｎ ｂｌｏｔｔｉｎｇ和电镜观察证实，表达产物是直径为２２ｎｍ的颗粒。

The coding sequencese of the hepatitis B virus (adr) surface antigen were inserted in the genome of BmNPV to construct recombinant BmNPV which were used to infect silkworm cells larvae and pupae. The level of HBsAg synthsis in cell cultures infected with the recombinant BmNPV was estimated at 35.5ug/ml cell culture (106cell), 750ug per larva and 690ug per pupa. Western blot and electronmicroscope show that primarily purified products are 22nm (diameter) glycosylated particles. It's buoyant density is 1. 2g/ml which is consistent with that of HBsAg in the plasma of patients.