抗黄曲霉毒素B_1单克隆抗体的制备及特性

Preparation and Characterization of Monoclonal Antibodies to Aflatoxin B_1

用杂交瘤技术制备了５株产生抗黄曲霉毒素Ｂ１单克隆抗体的杂交瘤细胞株。对其中之一ＡＦＢ１－２Ｈ８进行了较系统的研究。ＡＦＢ１－２Ｈ８属ＩｇＣ３。纯化腹水抗体效价约５×１０＾６。ＥＬＩＳＡ检测标准毒素的线性范围为０．５￣５０ｍｇ／ｍｌ。最低检出量为０．０１ｎｇ／ｍｌ。该单抗与参试的其它黄曲霉代谢物的交叉反应系数为０￣０．２１，该抗体有较大的应用价值。

Five hybridoma cell lines producing monoclonal antibodies (McAb) against aflatoxin B1 (AFB1) were established after fusion of mouse myecloma cells (Sp-2/0-Ag-14) with spleen cells isolated from male BALB/c mice immunized with AFB1-BSA conjugate. Among them, a McAb which was designated AFB1-2H8 was of the subtype IgG3 and the ascitic fluid of it gave suitably high dilution titres (1: 5X106). The sensitivity of a competitive enzyme- (linked immunosorbent assay (ELISA) using AFB1-2H8 for AFB1 was demonstrated that the linear range was 0. 5~50ng/ml and the minimum detectable concentration of AFB1 was 0. 01ng/ml. The specificity of the McAb was determined and it was shown no cross-rection significantly with any of the metabolites tested. So the McAb and the ELISA described may prove of use in the detection of AFB] foods and feeds.