钙调素化学发光标记的研究

Study on the Chemiluminescent Conjugatingof Calmodulin.

用发光物质Ｎ－氨基丁基Ｎ－乙基异鲁米诺（ＡＢＥＩ），采用碳二亚胺缩合法，成功地标记了动物及植物钙调素（ＣａＭ），标记率为［ＡＢＥＩ］／［ＣａＭ］≈０．８—０．９。对ＡＢＥＩ标记ＣａＭ的反应条件、结合物的质量和保存时间进行了观察。此标记物稳定，－２０℃可保存１０个月以上，适用于化学发光免疫法测定生物样品中ＣａＭ含量。

Animal and plant calmodulin wereconjugated successfully with the emission sub-stance,N-(4-aminobutyl)-N-ethyl-isoluminol(ABEI)by the method of carbodi-imide con-densation.The labelling rate of[ABEI]/[CaM]is 0.8 0.9.The reacting condition ofCaM with ABEI,the quality and the keepingtime of the ABEI-CaM conjugate were ob-served.The conjugate was stable and can bekept more than 10 months at -20℃. It wassuitable for the chemiluminescent immunoas-say in which the level of CaM in the biologicalsample was measured.