在96孔板中进行抗脂质过氧化的微量测定

One-Step Microassay for the Measurement ofAnti-Lipid Peroxidation in 96-Wells Plate.

以Ｆｅ￣（２＋）／半胱氨酸诱导大鼠肝微粒体为基本模型，根据硫代巴比妥酸（ＴＢＡ）反应原理，优化不同反应条件，建立了一种在９６孔板上进行抗脂质过氧化测定的一步反应方法，该方法的灵敏度不低于传统的试管法，而且还具有微量、快速、简便等优点，特别适用于大规模筛选和研究抗氧化剂。此外，也可用于其它系统诱导的抗脂质过氧化的测定。

Based on the mode of formation ofmicrosomal lipid peroxdation induced byFe￣(2+)/cysteine and the principle of TBA reac-tion,a simple one-step microassay for thestudying and screening of antioxidants in 96-wells plate was established. The comparativestudies between the procedures in 96-wellsplate and in test tube were also conducted.This microassay requires only small volume ofdiluted microsome and tested compounds,having the advantages of reliability,rapid-ness,simplicity and convenience. It is espe-cially suitable for the pilot screening of antiox-idants as well as the simultaneous study formany samples. This method can also be usedto measure the lipid peroxidation induced byother systems.