胃肠道肿瘤TGF－α基因表达的研究

Investigation of Expression of TGF α mRNAin Human Gastrointestinal Carcinoma.

用分子杂交技术及流式细胞方法研究了转化生长因子α（ＴＧＦα）在人胃肠道肿瘤组织中的表达．结果表明，胃癌、结肠癌和直肠癌组织较相应癌旁组织ＴＧＦαｍＲＮＡ水平增高．佛波脂（ＴＰＡ１００μｇ／Ｌ）作用胃癌细胞ＭＧｃ８０－３后４ｈ，可使细胞中ＴＧＦαｍＲＮＡ水平增高，作用６ｈ可使Ｓ期细胞百分比明显增加．这些结果提示：ＴＧＦα在胃肠道肿瘤中起重要作用，其表达可受ＴＰＡ的调节．

The expression of transforminggrowth factor α(TGFα)in human gastroin-testinal carcinoma by methods of molecularhybridization and cell FIT cell-cycle analysiswas investigated.The results show that thelevels of TGFα mRNA are higher in gastriccarcinoma tissues,colonic carcinoma tissuesand rectal carcinoma tissues than in the corre-sponding control tissues. After treating with12-O-tetradecanoylphorbol-3-acetate(TPA,100μg/L,4h),the level of TGFαmRNA ingastric carcinoma cell line(MGc80-3)increas-es, and percentage of S phase cells is marked-ly increased after 6 hours.These results sug-gest that TGF α plays an important role ingastrointestinal carcinoma tissues and expres-sion of TGFα mRNA can be regulated byTPA.