多药抗药基因Mdrl探针的克隆及初步应用

Cloning of Mdrl Gene Probe.

多药抗药基因Ｍｄｒｌ的表达水平与细胞的耐药性直接相关，检测Ｍｄｒｌ的表达水平可预测化疗的效果以及预后，用分子原位杂交的方法可检测单个细胞中Ｍｄｒｌ的表达水平．用ＰＣＲ扩增方法获得了一段特异的ＤＮＡ片段，并将其克隆到ｐＵＣ１８载体中，经ＤＮＡ序列分析证明与文献报道一致，此探针可用于临床标本的分子杂交检测．

Mdrl gene contributes to multidrugresistance.Because of the level of Mdrl geneexpression is correlated with the response ofchemotherapy,it is a valuable method to mea-sure the expression level of individual pa-tients. The cDNA cloning of the human Mdrlgene has made it possible to measure levels ofMdrl RNA with hybridization in human can-cer cells. A special part of Mdrl cDNA wasobtained by use of RT-pCR and inserting itinto pUC18 vector.The DNA sequencingresult is identical with the sequence of MdrlcDNA reported.This probe can be used to de-tect the level of Mdrl gene expression in clini-cal specimen,The measurements can be usefulin the design of chemotherapeutic protocolsfor certain tumors.