IL－2部分拮抗剂可用于IL－2突变体对受体亚基结合能力的初步鉴定

Primary Determination on Receptor SubunitBinding Ability of IL-2 Mutants by IL-2 Par-tial Antagonists,

用定点突变法分别得到了两个人白细胞介素－２（ＩＬ－２）的部分拮抗剂１５Ｖａｌ－ＩＬ－２和１２６Ａｓｐ－ＩＬ－２以及一个为ＩＬ－２受体α亚基结合缺陷型的突变体６２Ｌｅｕ－ＩＬ－２，当将１５Ｖａｌ－ＩＬ－２或１２６Ａｓｐ－ＩＬ－２与６２Ｌｅｕ－ＩＬ－２共同保温时，６２Ｌｅｕ－ＩＬ－２的活性受到明显抑制，对此现象机理的分析表明１５Ｖａｌ－ＩＬ－２或１２６Ａｓｐ－ＩＬ－２可用于ＩＬ－２受体亚基结合缺陷型突变体的初步鉴定．同时，这一思路在其它受体－配基系统中具有一定的适用性．

Two partial antagonists of humaninterleukin-2(IL-2):15 Val-IL-2,126Asp-IL-2 and an IL-2 receptor a subunit binding defi-cient mutant 62Leu-IL-2have been obtainedby site-directed mutagenesis, 62Leu-IL-2could exhibit significant stimulation on CTLL-2cells. When 15Val-IL-2 or l26Asp-IL-2 wasincubated together with CTLL-2 cells in addi-tion to 62Leu-IL-2,the stimulative ability of62Leu-IL-2 was distinctly inhibited.In con-sideration with the interaction of IL-2 with IL-2 receptor subunits,15Val-IL-2 or l26Asp-IL-2 can be used for the primary determina-tion on receptor subunit binding ability of IL-2mutants,Furthermore,this idea may also beapplied in some other ligand-receptor sys-tems.