RNase保护法同时检测黄体生成素受体及17α－羟化酶的mRNA

Simultaneous Detection of 17α-hydroxylaseand Luteinizing Hormone Receptor( LHR)mRNA from Rat Ovary by Ribonuclease Pro-tection Assay(RPA).

睾酮是组织中主要雌激素雌二醇的前体，在卵巢内睾酮绝大部分转为雌二醇．睾酮的生成直接受垂体的黄体生成素和睾酮合成的限速酶１７α羟化酶的调控．用液相杂交的方法，同时检测了卵巢组织中黄体生成素受体和１７α－羟化酶的基因表达，并摸索出了最适条件．为今后进一步研究卵巢的功能，提供了新的手段．

Testosterone is prehormone ofestradiol and most of it will converted toestradiol in ovary. The production of testos-terone was regulated directly by luteinizinghormone and 17α-hydroxylase which is therate-limiting enzyme in the production oftestosterone.So 17α-hydroxylase and LHRgene expression reflec the response to LH ofovarian cells. In order to investigate the ova-rian functions, the 17α-hydroxylase and LHRgene expression was detected simultaneouslyby using ribonuclease protection assay(RPA)and the sensitivity Of RPA is about 8 timesmore compared with Northern blot.