缺氧小鼠脑中神经节苷脂和单胺类神经递质水平

The Analysis on the Level of Gangliosides andMonoamine Transmitters under RepeatitiveHypoxia of Mice.

脑缺氧和缺氧后的再灌注常导致一系列复杂的生理生化改变．通过小鼠重复缺氧后，观察其脑组织中的神经节苷脂和单胺类递质水平变化情况，发现重复缺氧时，随着缺氧次数的增加，ａ．神经节苷脂水平（以唾液酸含量表示）非常明显地持续下降（Ｐ＜０．０１），其中ＧＭ１与ＧＤ１ｂ相对组分百分比值下降尤为突出（ＧＭ１：Ｐ＜０．０５，ＧＤ１ｂ：Ｐ＜０．０１）；ｂ．单胺类神经递质中ＮＥ和ＤＯＰＡ水平下降，ＤＡ和ＨＩＡＡ水平升高（Ｐ＜０．０１）．结果提示脑组织经重复缺氧后，中枢神经组织细胞膜受到一定程度的损伤，其结果可能影响到单胺类神经递质的合成、释放、重摄取和贮存的全过程；据此推测脑组织缺氧时神经节苷脂与单胺类神经递质水平改变时存在着相互联系．

Hypoxia and post-hypoxia reinfu-sion usually lead to a series of complicatedphysiological and biochemical changes.Through the model of mouse repetitivehypoxia ,the content changes of gangliosidesand monoamine neurotransmitters were ob-served in the brain and it was found that:withthe increment of the anoxic times:①Ganglio-sides(calculated as NANA)was continuouslyfalling(P<0.01),and the falling of relativepercentages of GM1 and GD1b of which weremagnificent(GM1:P<0.05,GD1b:P<0.01);②Among monoamine neurotrans-mitters,the level of NE and DOPA descendedand that of DA and HIAA increased(P<0.01).The results point out:after repetitivehypoxia,the cell membrane in centrical nervetissues is injured in some degree and thus mayaffect the whole process of synthesizing,re-leasing, reabsorbing and storing ofmonoamines. There is association relation be-tween changes of gangliosides andmonoamines when hypoxia occurs.