碱基上的氨臂对T_4多核苷酸激酶催化的影响

Influence of the Amino-Space Modification ofa Base in an Oligonucleotide on the Activity ofT_4 Polynucleotide Kinase.

寡聚核苷酸内部和５′末端含有的氨臂修饰胸苷酸，能够明显地影响Ｔ＿４多核苷酸激酶催化的［γ－￣（３２）Ｐ］ＡＴＰ的转移反应．其转移效率为未修饰寡聚核苷酸的５０％和２％．这种修饰的寡聚核苷酸对Ｔ＿４ＲＮＡ连接酶催化的反应没有影响．

It was found that the presence of anamino-space modifying thymidine in anoligonucleotide interfered the transfer reactionof [γ-32￣P] ATP bv T_4 polynucleotide kinase.When this modified thymidine located in themiddle of oligonucleotide,50%activity of T_4polynucleotide kinase was inhibited.Whenthis modified thymidine located at the 5'-ter-minus,only 2%activity remained.Compara-tively,those modifications did not affect theactivity of T_4 RNA ligase.