摘要
按Laarse's方法建立培养心肌细胞缺氧-再给氧(A-R)模型,缺糖缺氧60min,再给氧30min.结果发现,缺氧组心肌细胞MDA含量增加,SOD活性降低,细胞膜脂质流动性下降,再给氧组上述改变加剧.酸枣仁总皂忒(ZS)能剂量依赖性地显著降低心肌细胞MDA含量,提高SOD活性,增加细胞膜脂质流动性,证明ZS有明显抗心肌细胞缺氧-再给氧损伤作用.
The anoxia-reoxygenation model ofcultured neonatal rats myocardial cells weredeveloped, according to Laarse's method :anoxia and glucose deficiency for 60 min fol-lowed by reoxygenation and re-exposure toglucose for 30 min.The results showed thatthe intracellular malondialdehyde (MDA)con-tent in the anoxia group was significantly in-creased,superoxides dismutase(SOD)activitywas obviously reduced and membrane fluidity was decreased,all the changes were muchmore severe in the reoxygenation group.Totalsaponins of semen Ziziphi spinosae(ZS)couldmarkedly and dose-dependently decrease MDAcontent,elevate SOD activity and increasemembrane fluidity.It proved the effects of ZSagainst lipid peroxidation induced by anoxia-reoxygenation.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1995年第6期540-542,共3页
Progress In Biochemistry and Biophysics
关键词
心肌缺氧
再灌注损伤
酸枣仁总皂甙
药理学
total saponins of semen Ziziphisaponase, anoxia-reoxygenation,cultured my-ocardial cells,malondiaidehyde(MDA),su-peroxide dismutase(SOD),membrane fluidity