酸枣仁总皂甙对缺氧－再给氧心肌细胞的保护作用

Protective Effects of Total Saponins of SemenZiziphi saponase on Cultured Myocardial CellsExposed to Anoxia-Reoxygenation.

按Ｌａａｒｓｅ＇ｓ方法建立培养心肌细胞缺氧－再给氧（Ａ－Ｒ）模型，缺糖缺氧６０ｍｉｎ，再给氧３０ｍｉｎ．结果发现，缺氧组心肌细胞ＭＤＡ含量增加，ＳＯＤ活性降低，细胞膜脂质流动性下降，再给氧组上述改变加剧．酸枣仁总皂忒（ＺＳ）能剂量依赖性地显著降低心肌细胞ＭＤＡ含量，提高ＳＯＤ活性，增加细胞膜脂质流动性，证明ＺＳ有明显抗心肌细胞缺氧－再给氧损伤作用．

The anoxia-reoxygenation model ofcultured neonatal rats myocardial cells weredeveloped, according to Laarse's method :anoxia and glucose deficiency for 60 min fol-lowed by reoxygenation and re-exposure toglucose for 30 min.The results showed thatthe intracellular malondialdehyde (MDA)con-tent in the anoxia group was significantly in-creased,superoxides dismutase(SOD)activitywas obviously reduced and membrane fluidity was decreased,all the changes were muchmore severe in the reoxygenation group.Totalsaponins of semen Ziziphi spinosae(ZS)couldmarkedly and dose-dependently decrease MDAcontent,elevate SOD activity and increasemembrane fluidity.It proved the effects of ZSagainst lipid peroxidation induced by anoxia-reoxygenation.