Gc、Pi蛋白亚型的快速微量电泳分析

Subtyping of Gc and Pi System by Rapid andSensitive Isoelectrofocusing.

用快速微量等电聚焦技术对１９０名北京地区汉族健康人血清Ｇｃ蛋白亚型、Ｐｉ蛋白亚型进行分型鉴定和基因频率调查．上样量为１．５μｌ，电泳和染色各０．５ｈ．Ｇｃ￣（１Ｆ）＝０．４８９１，Ｇｃ￣（１Ｓ）＝０．２４３２，Ｇｃ￣２＝０．２６７８．观察值与期望值吻合良好．（∑Ｘ￣２＝１．４０４，０．７＜Ｐ＜０．８）．Ｐｉ￣（Ｍ１）＝０．７５４２，Ｐｉ￣（Ｍ２）＝０．１８０８，Ｐｉ￣（Ｍ３）＝０．０６５０，观察值与期望值吻合也良好，（∑Ｘ￣＝１．１２３３，０．７＜Ｐ＜０．８）．

The distribution of Gc and Pi sub-types of 190 unrelated healthy donors in theHan population in Beijng were investigated byrapid and sensitive isoelectrofocusing. Therunning and developing time was half an houreach.Sample loading was 1.5μl Gc￣(1F)isO.4891,Gc￣(1S)is 0.2432 ,Gc￣2 is 0.2678.Thereis good agreement between the observed andthe expected value (∑X￣2=1. 404,0.7<P<0.8).Pi￣(M1)is 0.7542, Pi￣(M2)is 0.1808, Pi￣(M3)is0.0650.The agreement is good as well(∑X￣2=1.1233,0.7<P<0.8).