新型生长抑肽对成纤维细胞的DNA合成及某些癌基因表达的影响

Effect of a Novel Negative Growth Peptide on DNA Synthesis and Some Oncogenes Expression in Fibroblast

从细胞水平和基因水平研究了新型生长抑肽（ＥＰＰ）的生物学作用，研究表明：ＥＰＰ对ＮＣ３Ｈ１０及ＴＣ３Ｈ１０细胞的ＤＮＡ合成均有抑制作用，当ＥＰＰ与ｃＡＭＰ的位点选择性类似物８－Ｂｒ－ｃＡＭＰ共同作用时，其对ＮＣ３Ｈ１０细胞的ＤＮＡ合成的抑制作用消失，而对ＴＣ３Ｈ１０仍具有抑制作用；核酸杂交分析表明，ＥＰＰ可以抑制ｃ－ｆｏｓ、ｎｅｕ、ｋｉ－ｒａｓ三类癌基因在转化细胞中的表达。证明了ＥＰＰ对转化细胞的生长具有一定的抑制效应，且与８－Ｂｒ－ｃＡＭＰ联合使用时其效果更佳。

The epidermal pentapeptide (EPP) was able to inhibit incorporation of 3H-TdR into normal and transformed fibroblast(NC3H10 and TC3H10).When both EPP and 8-Br-cAMP acted on NC3H10 and TC3H10,they still inhibited incorporation of 3H-TdR into TC3H10, but no obvious effect to NC3H10 was shown.The results of blots showed that the expression of cfos, ki-ras, and neu in TC3H10 decreased after the cells were treated by EPP.So we think that the negative growth peptide may have anti-tumor effect, particularly it is used with 8-Br-cAMP together.