摘要
乙肝前Ｓ２（ＨＢＶＰｒｅＳ２）肽段由５５个氨基酸组成，其Ｎ端肽段含Ｔｈ和Ｂ细胞抗原决定簇，为增强ＰｒｅＳ２的抗原性，本实验采用将化学合成的ＰｒｅＳ２ｅｐｉｔｏｐｅ（１２０－１４５）基因以串联方式与ＨＢｃＡｇ的基因进行了融合，融合基因在大肠杆菌中获得表达，并通过ＥＬＩＳＡ比较研究了融合蛋白中ＰｒｅＳ_２ｅｐｉｔｏｐｅ单体及串联体的抗原性差异。
HBV PreS2 consists of 55 amino acids, 26 amino acids at N terminal are the Th cell and B cell epitopes.In order to enhance the antigenicity of PreS2 epitope, singal and tandem repeats of PreS2 epitope genes were frused with HBcAg gene. The genes could be expressed in E. coli and the antigenicity of these fusion proteins were detected comparatively by ELISA.
