天然和氧化修饰型LDL、VLDL及HDL对培养人动脉平滑肌细胞原癌基因fos及myc表达的影响

Effect of Native and Oxidization-Modificd LDL,VLDL and HDL on Transcriptional Expression of fos,myc Oncogenes of Cultured Human Arterial SMC

动脉平滑肌细胞（ＳＭＣ）是动脉粥样硬化（ＡＳ）斑块中的主要细胞，它的增殖在ＡＳ形成过程中极其重要。脂蛋白和氧化修饰型脂蛋白对ＳＭＣ增殖的影响以及ＳＭＣ增殖与原癌基因异常表达的关系是当前ＡＳ发病机制研究的热点之一。我们在建立人主动脉ＳＭＣ体外培养方法的基础上，观察了ＬＤＬ，ＶＬＤＬ及ＨＤＬ和相应的氧化修饰型脂蛋白对培养人ＳＭＣｆｏｓ，ｍｙｃ，ｅｒｂ－Ｂ原癌基因转录表达的影响。结果表明：①ＨＤＬ对ＳＭＣｆｏｓ，ｍｙｃ基因表达无影响；②ＬＤＬ和ＶＬＤＬ有使这些基因表达增加的趋势，但与对照比较差异不显著（Ｐ＞０．０５）；③ＯＸ－ＶＬＤＬ，ＯＸ－ＶＬＤＬ和ＯＸ－ＨＤＬ有使ＳＭＣｆｏｓ，ｍｙｃ基因表达显著增强的作用（Ｐ＜０．０１），且其作用较相应的天然脂蛋白大（Ｐ＜０．０１）．上述结果说明：ＬＤＬ，ＶＬＤＬ，ＯＸ－ＬＤＬ，ＯＸ－ＶＬＤＬ和０Ｘ－ＨＤＬ的致ＡＳ作用可能与刺激ＳＭＣｆｏｓ和ｍｙｃ癌基因表达增加有关。

The smooth muscle cells (SMCs) are the predominant type of cells within atherosclerotic lesions,and their proliferation plays an important role in the process of AS genesis. On the basis of the establishment of primary culture and sub-culture method for human arterial SMC,we observed the effects of LDL,VLDL,HDL,OX-LDL,OX-VLDL and OXHDL on oncogene transcriptional expression of cultured SMCs. Results were the followings: 1. HDL had no effects on the expression of fos,myc and erb-B genes in cultured human SMC. 2. LDL and VLDL induced slight increase in the expression of those genes respectively,but the increase was insignificant (P>0. 05). 3. OX-LDL, OX-VLDL and OX-HDL promoted the expression of those oncongenes significantly (P<0. 01),and the promoting effects were greater than those of the corresponding native lipoproteins(P<0. 01). These results suggest that the atherogenic role of LDL,OX-LDL,OX-HDL and OX-VLDL are closely related to their stimutating effects on proto-oncogene expression.