摘要
对ＲＡ、ＨＨＴ和ＷＰ_（８５２）诱导ＨＬ－６０细胞分化过程中胞浆和膜溶脱部分中的ＴＰＫ和ＰＴＰＰ活力变化进行了研究．结果表明，在诱导早期，ＴＰＫ的活力就有明显的波动变化；随着诱导时间的延长，胞浆部分ＴＰＫ活力下降，膜溶脱部分的ＴＰＫ活力则升高．诱导后，胞浆部分和膜溶脱部分的ＰＴＰＰ活力均明显升高。与ＴＰＫ活力变化相比较，ＰＴＰＰ变化幅度比ＴＰＫ的大。
Alternations of TPK and PTPP activities in cytosol and membrane fractions were studied during the differentiation of HL-60 cells induced by RA. HHT and WB_(852). It was found that the activity of TPK showed a distinct fluctuation in the early stage of differentiation and with the elongation of differentiation,it declined in cytosol fraction and increased in membrane fraction. It was also found that the activity of PTPP increased distinctly both in cytosol and memerane fractions. The range of increase was greater than the range in change of TPK activity.
基金
国家自然科学基金
关键词
人早幼粒白血病
蛋白激酶
TPK
白血病
细胞分化
Human promyelotic leukemia cell line (HL-60)
Retinoic acid (RA)
Homoharringtonine(HHT)
WB_(852)
Tyrosine protein kinase (TPK)
Phosphotyrosyl protein phosphatase (PTPP)
