摘要
用C14(DCCD)参入法确定了在泛醌结合蛋白QPs中有四个对重组活性必需的羧基,它们全部定位在24000亚基上。
It was proved in the last paper that the DCCD can modify the carboxyl groups of QPs leading to the lose of its activity of reconstitution with succinate dehydrogenase.C14 (DCCD) was used to incorporate into QPs and estimated that four carboxyl groups were essential for its reconstitution and all the four carboxyl groups were located in the subunit with molecular weight 24000.
关键词
泛醌结合蛋白
重组活性
化学修饰
羧基
Q-binding protein, reconstituted activity ,chemical modification