摘要
应用原位分子杂交技术及细胞图象分析证实了人肝癌细胞株ＳＭＭＣ７７２１细胞经１０μｇ／ｍｌ的ＧＭ_３／ＧＤ_３处理后，ＧＤ_３处理组细胞内Ｎ－ｒａｓ基因ｍＲＮＡ的阳性信号弥漫分布于整个细胞，而经ＧＭ_３处理组和对照组阳性信号集中于核膜。ＧＭ_３／ＧＤ_３处理组及对照组ｃ－ｆｏｓ基因ｍＲＮＡ的阳性信号均集中于核膜，但ＧＭ_３处理组信号强度最大。上述结果提示，ＧＤ_３可使某些肿瘤细胞向更加恶性的方向发展，而ＧＭ_３则可使某些肿瘤细胞向正常方向转化。
With the method of in situ hyridyzation and cell image analysis, it was showed that the positive signal of N-ras mRNA in the GD_3-treatcd cells distributed broadly within the whole cell, whereas that of GM_3-treated and control cells were concentrated on the nucleus membranes. The positive signals of c-fos mRNA in nucleus membranes, but GM_3-treated cells has the strongest one. The results mentioned above indicated that GD_3 could induce some tumer cells to be more malignant, but GM_3 could reverse some of the effect.
基金
国家自然科学基金
