摘要
利用缺口修复（ｇａｐｒｅｐａｉｒ）方法克隆啤酒酵母（Ｓ．ｃｅｒｅｖｉｓｉａｅ）野生型ＲＡＤ_（２４）基因，并将其亚克隆到Ｍ１３ｍｐ１８和Ｍ１３ｍｐ１９，用双脱氧末端终止法对该基因的两条链均进行了序列测定。ＤＮＡＳｔｒｉｄｅｒ程序分析显示该基因编码２６８个氨基酸的蛋白质；基因缺失试验表明，该基因为细胞生存所必需。
A wild type RAD_(24) gene of S. cerevisiae was cloned by gap repair and subcloned into M13mp18 and M13mp19. Both strands of the gene were sequenced by the Sanger dideoxymediated chain-termination method. The result programed by DNA Strider indicated that the cloned gene encodes a protein of 268 amino acids. Deletion of of this gene in genome is lethal,Showing that RAD_(24) is an essential gene.
