Ap—2号溶磷黑曲霉深层发酵工艺的研究

The Studies of the Technology of Deep Fermentation Processes of Ap- 2 Dissolve Phosphorus Aspergillus Niger

为了实现Ａｐ—２号磷菌肥的工业化生产，对Ａｐ－２号溶磷黑曲霉进行了深层发酵工艺的研究。确定了最佳发酵条件：Ｃ／Ｎ１０：１；葡萄糖４％，黄豆饼粉２％，麸皮１％，酵母粉０．５％，ＭＰＳＯ４·７Ｈ２Ｏ０．０５％，Ｋ２ＨＰＯ４０．１％，温度３０℃，ＰＨ６，摇床转数１８０ｒ／ｍｉｎ．发酵９６小时，ｐＨ降至３．１，残糖降至１％以下．柠檬酸、草酸总量达３％以上，菌丝浓度达２．５９／１００ｍｌ。采用ＥＳ—１５型自控发酵罐扩大培养，与摇床培养结果相符。

In order to commercialize AP--2 phosphobacteria manure , the author studied the technology of deep fermentation processes of AP -- 2 dissolve phosphorus aspergillus 'iger. Thepotimum coditious of the fermentation were determined: C/N 10: 1, glucose 4%, Soybeancake powder 2%, bran 1% yeast powder 0. o%, MgSO4. 7H,O 0. 05%, K2HPO.40. 1%,temperature 30℃, PH 6, rotation speed of universal shaver table 180r/min. After the fermentation for 96 hours. PH fen into 3. 1, survival sugar fell into<1 %, quantity of citric acidand oxalic acid was more than 3% , the concentration of hyphae was 2. 5g/100ml. The enlarge culture was carried out in ES-- 15 type automatic fermentation tank, and the result corresponded with the culture result in universal chaker table.