用共价偶联方法研制压电晶体免疫传感器

DEVELOPMENT OF A PIEZOELECTRIC IMMUNOSENSOR BY COVALENT BINDING METHOD

以牛血清蛋白（ｂｏｖｉｎｅ ｓｅｒｕｍ ａｌｂｕｍｉｎ，ＢＳＡ）及其抗体为检测对象，用常用的硅烷化合物ＡＰＴＥＳ固定蛋白研制石英晶体免疫传感器。测定了ＢＳＡ在石英晶体上的固定饱和曲线及甘氨酸（Ｇｌｙ）对戊二醛的封闭效应。特异性结合的ＢＳＡ可以用０．２ｍｏｌ／Ｌ柠檬酸洗脱，抗ＢＳＡ抗体膜可以重复使用，但其活性下降。

A piesoelectric immunosensor for detection of BSA and anti-BSA antibody, using APTES to immobilize the protein, is described. The optimum concentration of BSA used for immobilisation on the surface of the quartz Crystal and the blocking affeCt of the Gly to glutaraldehyde are tested. BSA spedfically binding to anti -BSA antibody can be removed by dtric add (0.2mol/L). The antibody coated Crystal can be used repeatedly, but its activity decreases.