Ebselen和谷胱甘肽对低密度脂蛋白氧化修饰的抑制和终止作用

INHIBITION AND TERMINATION EFFECT OF EBSELEN AND GSH ON THE OXIDATIVE MODIFICATION OF LOW DENSITY LIPOPROTEIN

研究了人工合成的谷胱甘肽过氧化物酶（ＧＳＨＰｘ）模拟物－ｅｂｓｅｌｅｎ及其辅助因子谷胱甘肽（ＧＳＨ）对Ｃｕ２＋及紫外线（ＵＶ）诱导的低密度脂蛋白（ＬＤＬ）氧化修饰的抑制和终止作用。结果显示：在ＧＳＨ存在的情况下，低主５－μｍｏｌ／Ｌ的ｅｂｓｅｌｅｎ就可完全抑制由５μｍｏｌ／ＬＣｕ２＋引发的ＬＤＬ氧化修饰，单独的ｅｂｓｅｌｅｎ和ＧＳＨ没有抑制作用，５０μｍｏｌ／Ｌ的维生素Ｅ只有很微弱的抑制作用。Ｅｂｓｅｌｅｎ对Ｃｕ２＋引发的ＬＤＬ氧化修饰的抑制作用时间的长短与ＧＳＨ的浓度有关。对已由Ｃｕ２＋引发启动的ＬＤＬ氧化修饰，加入ｅｂｓｅｌｅｎ和ＧＳＨ可防止其进一步的氧化。Ｅｂｓｅｌｅｎ和ＧＳＨ对紫外线引起的ＬＤＬ氧化修饰也有一定的抑制作用。

Inhibition and termination effect of a synthesized glutathione perendase rnnmc-ebselenand its cofactor GSH on the oxidative moderation of lOw density lipoproch (LDL) studied by detemnination of thiobarbituric and reactive substance gnase) and fluorescence intensity. The results showed that ebselen at a low concentration of 5μmol/L could inhibitoxidative modistion of LDL induce by saal/L Cu2+ completely in the presence of GSH(50μmol/L). Neither ebselen nor GSH alone had the effat, Vitamin E (50μmol/L) had only avery weak effed. The the of inhibition of ebeelen on the oxidative modistion of LDL induced by Cu2+ was dependent on the conCentlation of GSH. Ebselen together with GSH couldprevent further oxalation of LDL. As to the oxidahve rnodfotion of LDL induce by UVinadtation, to some extent an inhibitory effat of ebeelen and GSH was shown.