摘要
本文以透析、离子交换层析及聚丙烯凝胶电泳等手段从孕牛血清中分离纯化早孕因子(EPF).EPF的活性用玫瑰花环抑制实验确定,其分子量用SDS—PAGE法测定,含量用LOWry方法测定.
出处
《生物学杂志》
CAS
CSCD
1995年第3期18-19,共2页
Journal of Biology
基金
国家自然科学基金
同被引文献37
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二级引证文献11
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1王晓申,魏丽军,叶绍辉.早孕因子研究进展[J].生物技术通讯,2008,19(2):313-316. 被引量:5
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3练玉银,王家骥.重组早孕因子(EPF)的表达、纯化及鉴定[J].生殖与避孕,2012,32(3):156-160. 被引量:4
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6张兴会,宋先忱,郭丹,韩迪.绒山羊早孕因子的活性检测及分离纯化[J].现代畜牧兽医,2013(11):8-12. 被引量:1
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7郑玮璐,刘为民,陈芳,王丙云,梁梓森,计慧琴,谢莹佳.Hela细胞早孕因子基因的克隆与原核表达[J].动物医学进展,2015,36(5):11-15.
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1孙文东,吴瑞英,邓雁如,赵明,努尔.波拉提.孕牛血清中早孕因子的生化特性研究[J].新疆医科大学学报,2003,26(3):222-223. 被引量:2
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