摘除松果体对大鼠运动活动近似昼夜节律的影响

The Effects of Removing both Pineal Gland and Eyes on the Circadian Locomotor Rhythm in rats

为了分析松果体及其褪黑激素在哺乳动物近日节律调控机制中的作用，本文对摘除松果体和眼球后的大鼠的运动活动，进行了连续６天的观测，并以单纯致盲的动物作对照。经用余弦节律分析法对实验数据进行处理，结果表明．与对照组相比，去松果体的各动物运动活动昼夜节律的峰值相位较分散，节律振幅的算术平均值较小；对群体平均的节律特征值进行统计学检验，见去松果体动物群体平均的运动活动已无明显的昼夜节律而对照动物的此节律仍然存在。根据上述结果。作者认为，在哺乳动物近日节律的内源性调控机制中，松果体仍有积极作用，其作用在于增大节津振幅和导引节律时相。

Activities of rats were observed after removal of their pineal gland and eyes for six consecutive cays. being blinded rats were used as controls. Characterizing parameters of rats' circadian locomotor rhythm such as amplitude and acrophase were analysed with consinor-rhythmometry. The result showed, by observing each rat separately. individuals in the two groups were obviously different in extent in circadian rhythm. Compared with control group, individuals among tested group had greater differences,especially in rhythmic acrophases. The arithmetic average of rhythmic amplitude of individual in the tested group was smaller than that in the control group. The characterizing parameters of population rhythm of the rwo group animals were calculated with population mean-cosinor; the amplitude of the tested group was obviously smaller than that of the control group. Statistical tests suggested the population of the tested group should have no distinctive circadian rhythm, but the control group obviously have. According to the above, we believe that pineal gland plays an important role in increasing amplitude and entraining rhythmic phase of circadian locomotor rhythm though it is not a determinative circadian rhythmic oscillator of mammal.