输精管结扎家兔外周血淋巴细胞丝裂原反应性、IL－2及巨噬细胞IL－1活性的动态研究

Dynamic Observation of the Influence of Vasectomy on Mitogenesis of T Lymphocytes、IL-2 and IL-1 Activity of Peripheral Blood from Vasectomized Rabbits

体重２．５～３．０ｋｇ的日本大耳白雄兔２８只，分为输精管结扎组（ＶＧ，１８只）和假手术对照组（ＳＯＧ，１０只）。ＶＧ行双侧输精管结扎手术，ＳＯＧ只行双侧输精管暴露手术，术后第４、８、１２、１８、２２月检测外周血淋巴细胞对丝裂原反应性、ＩＬ—２和巨噬细胞ＩＬ－１活性。结果表明：１．ＣｏｎＡ和ＰＨＡ诱导的淋巴细胞丝裂原反应性，术后第８月ＶＧ的３Ｈ—ＴｄＲ掺入量均明显低于明Ｇ（Ｐ＜０．０１），而术后第４、１２、１８、２２月的测定结果与ＳＯＧ比较均无差异（Ｐ＞０．０５）。２．ＩＬ－２活性也以术后第８月为最低，与ＳＯＧ比较差异显著（Ｐ＜０．０１），１２月后恢复正常。３．ＣｏｎＡ诱导的淋巴细胞丝裂原反应性和ＩＬ－２活性间呈有意义的正相关关系（ｒ＝０．６６１，Ｐ＜０．００１）。４．ＩＬ－１活性，ＶＧ第８月显著地高于ＳＯＧ第８月（Ｐ＜０．０５），其它各月检测结果两组比较无明显差异。

To study the functional changes of immunological surveillance after vasectomy,we have dynamically observed the influence of vasectomy on the function of T lymphocytes and macrophages of peripheral blood from rabbits by using a model of animals which were vasectomized for 22 months. The results slowed that: (1) PHA and ConA induced mitogenesis of T lymphocytes from the rabbits vasectomized for eight months was significantly inhibited (P<0. 01) and recovered in rabbits vasectomized for 12. 18. 22 months respectively; (2) The IL-2 from peripheral mononuclear cells (PBMc) in the rabbits vasectomized for 8 months was reduced remarkably (P<0. 01)and reached normal level in the rabbits vasectomized for 12 months; (3) There was a significant correlation between mitogenesis of T cells induced by ConA and the levels of IL-2 from PBMc.(4) IL- 1 secretion from peripheral blood macrophages induced by LPS in the rabbits vasectomized for 8 months increased remarkably (P<0. 02) and reached normal level in the rabbits vasectomized for 12 months. The results may provide an experimental evidence for the evaluating the function changes of immunological surveillance in vasectomized animals.