摘要
本文报道碱性α－淀粉酶１４Ｌ罐发酵工艺及其酶的性质。发酵过程中，当搅拌转速为４００ｒ／ｍｉｎ，通风比为１：１．２５Ｌ／（Ｌ·ｍｉｎ），发酵周期４４ｈ，酶活力可达７４０ｕ／ｍｌ。采用有机溶剂沉淀法提取酶，回收率可达９０％。Ｃａ￣（２＋）对酶活性无影响，ＥＤＴＡ对酶有激活作用。酶样保存半年，活力损失小于１５％。
he fermentation of alkaline amylase from the alkalophilic Bacillus megateriun L-49 in 14L tank has been studied. The optimum ventilation quantity and agitation speed were 1:1. 25 vvm and 400r/min respotivily.The fermentation cycle was 44h and the highest alkaline amylase activity was 740u/ml.The enzyme was recovered by precipitation method of organic solvents.The effects of surfatants,EDTA and Th2+ ion on enzymes activity were also studied.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
1995年第3期21-25,共5页
Food and Fermentation Industries
基金
天津市21世纪青年科技基金
