摘要
本文用紫外分光光度法测定大豆粉中的总皂苷。样品经脱脂、甲醇提取、正丁醇萃取分离后，在２８０ｎｍ处测定硫酸水解物的吸光度。实验表明，本法的回收率为９５～９９％。四次测定结果的相对标准偏差为６．６％。
otal saponin was determined by UV spectrophotometry. Sample was defated, estracted with methanol. After separation by n-butanol extraction. absorbance of the hydrolysate in sulfuric acid was determined at 280nm. It has been found that the recovery of this method is 95～99%. Relative standard deviation is 6. 6% for 4 measurements.
出处
《食品与发酵工业》
CSCD
北大核心
1995年第6期44-46,共3页
Food and Fermentation Industries
关键词
分光光度法
大豆
皂苷
大豆粉
测定
Spectrophotometry
Total soybean saponin
Soybean flour.
