摘要
竹红菌乙素与乙醇胺进行化学结构的修饰,可以得到红光性能更加优良的新型光敏剂(简称HB-E),为此,我们作了以下几方面的研究:1.对线粒体膜脂质过氧化损伤的光敏作用;2.对线粒体膜巯基蛋白光敏损伤;3.对ATP酶的失活;4.损伤机理的探讨;5.不同光敏剂光敏能力的对比;从以上研究可以看到HB-E光敏作用对于线粒体的损伤十分明显,从损伤的机理角度证明了氧自由基的作用是存在的,对于体系中产生超氧阴离子的来源认为并不是单线态氧的作用,而是HB-E自身产生自由基与氧反应的结果;所以说HB-E光敏作用中存在两种机制即:Ⅰ型和Ⅱ型并存;与其它光敏剂的光敏能力比较中明显大于血卟啉和亚甲兰。
The Hypocrellin B-Ethanolamine(HB-E) sensitized photodamage on lipid pero-xidation, sulfhydryl oxidation and ATPase inactivation of rat liver mitochondria was studied under illumination of 640 nm, The HB-E sensitized photodamage of lipid pe-roxidation is less than sulfhydryl photoo-xidation, when HB-E concentration is 50 μmol/L.
To elucidate the mechanism of photo-damage of HB-E, antioxidant: BHT, Vit E, oxygen radical quencher: L-His, β-
Cart, were investigated. The results showed that HB photodamage of mitochondria is not only by oxygen radical, but also by HB-E radical as well.
From compared tests among HB, HA, Hp, MB and HB-E, we found that HB-E had higher ability to photodamage lipid peroxidation, sulfhydryl oxidation and tryptophan than other photosensitizers. The experiments showed that the superox-ide generated may be by HB-E radical.
出处
《实验生物学报》
CSCD
1995年第1期23-30,共8页
Acta Biologiae Experimentalis Sinica
基金
国家自然科学基金
