人畸胎瘤细胞株PA-1的体外诱导分化

THE INDUCTION AND DIFFERENTIATION OF A HUMAN TERATOCARCINOMA CELL LINE (PA-1) IN VITRO

PA-1细胞是一株从人卵巢性畸胎瘤衍生的细胞株,经10^(-5)mol/L视黄酸诱导后,部分细胞的形态和排列方式发生一定的改变。通过细胞免疫荧光染色,发现诱导后细胞的结蛋白和胞外基质(纤粘连蛋白、层粘连蛋白和腱粘连蛋白)表达与分布模式有较大的变化,并且这些变化与细胞形态改变相关。但大部分PA-1细胞诱导后的生长状况并没有较大的改变,仍分泌与表皮生长因子、类胰岛素生长因子-Ⅰ相关的活性物质。以上结果提示PA-1细胞经视黄酸诱导后一部分细胞可能向肌细胞方向分化。

PA-1 cell line is derived from human ovary teratocarcinoma. When it grows in 10% fetal calf serum, it can be induced to differentiate by 10-5mol/L retinoic acid (RA). Some morphological changes can be observed after RA induction. By immuno-staining of cultured cells, we found that the expression and distribution pattern of desmin and some extracellular matrix mo- lecules, such as fibronectin, laminin and tenascin, had been changed after induction, and these changes were associated with the morphological changes. Cell growth study showed that RA treatment had no effects on growth and autocrine activities of PA-1 cells. These results suggest that some PA-l cells were induced to differentiate along muscle cells pathway by RA.