乳腺癌GST—л抗体免疫组织化学检测的研究

Detection of Anti GSt л Antibody by Immunohistochemical Technique in Mammary Cancer

应用抗人胎盘型谷胱甘肽转移酶（ＧＳＴ—л）抗体的ＡＢＣ免疫组织化学技术，测得正常乳腺组织１０例ＧＳＴ－л阳性率为１０．００％。１２例纤维腺瘤和８例腺病，ＧＳＴ—л阳性率分别为１６．３３％和１２．５０％，２０例异型增生组织的阳性率为９０．００％；４０例乳腺癌组织中阳性率为８５．００％。其中小叶原位癌导管、内癌、浸润性导管癌、浸润性小叶癌、单纯癌和髓样癌ＧＳＴ—л阳性率分别为７５．００％、５０．００％、９０．００％、８３．３３％、１００．００％和７５．００％。结果显示，ＧＳＴ—л抗体在乳腺癌和异型增生组织表达较高，而正常组织和良性病变较低，因此，ＧＳＴ—л抗体的免疫组化检测可作为乳腺癌的一项新的早期诊断指标。

Human placental glutathione S transferase(GST л)has been used to detect GST л activity in benign or malignant tissues of the breasts by avidin biotin peroxidase complex (ABC) methodwith anti GST лantibody immunohistochemical technique. Among 10 Samples of normal mammarygland tissues,positve GST лrate accounts for 10%. Among 12 samples of fibroadensma and 8 of adeno-sis,the positive rates,16.33%and 12.5%,respectively.Positive rate of 20 of hetrohyperplasia,90%.The positive rate of 40 cases of breast Carcinoma,85%,among which lobular carcinoma in situ, 75%;in-tradtuctal carcinoma,50%;infiltrating duct carcinoma,90%;infiltrating lobulur carcinoma,83.33%;simple carcinoma,100% and medullary carcinoma,75%. The results indicate that the expression of GST лantibodies is higher in breast cancer and heterohy-perplasia, and lower in the normal tissues and benign lesions,therefore, GST лis a new sensitive indexfor immuohistochemical detection of breast carcinoma in early stage.