环丙沙星对疟原虫子孢子活力的影响

EFFECT OF CIPROFLOXACIN ON PLASMODIUM SPOROZOITE DEVELOPMENT

约氏疟原虫子孢子与新型抗生素环丙沙星不同剂量共同孵育３０ｍｉｎ后腹腔接种昆明株小鼠体内．观察环丙沙星对子孢子活力的影响。环丙沙星分为１、２、４、６、μｇ／ｍｌ５种剂量，另外设双抗（青霉素十链霉素）对照组和ＰＢＳ对照组，每组接种５只小鼠，每鼠０．１ｍｌ，内含１０．５×１０４子孢子，３ｄ后尾静脉取血镜检，结果环丙沙星各试验药物组原虫血症出现的平均时间分别为３．８、３．６、３．８、３．８、３．２ｄ，双抗对照组和ＰＢＳ对照组均为３．８ｄ。结果提示环丙沙星剂量达８μｇ／ｍｌ与子孢子孵育３０ｍｉｎ后仍对子孢子活力无影响。

Sporozoites of Plasmodium yoelli having been stayed in 1, 2, 4, 6, 8ug/ml ciprofloxacin for 30 min were given intraabdominaly to Kunming mice for studying the effect on sporozoite development. There were also bi-antibiotic (penicillin+streptomycin) control and PBS control groups.Each group contained 5 mice and each mouse was inoculated 10. 5 × 104 sporozoites' The time of parasitemin in ciprofloxacin groups were 3. 8, 3. 6, 3. 8, 3. 8, 3. 2 d respectively, while they were 3. 8 d in both bi-antibiotic and PBS control groups. The results suggested that ciprofloxacin at 8ug/ml for 30 min revealed no effect on sporozoite development in mice.