深低温冷冻对降低异体骨抗原性作用的实验研究

Experimental study of deep-frozen on the decrease of antigenicity of allogenic bone.

应用现代免疫学技术,采用敏感的乳酸脱氢酶(LDH)释放法,测定了经深低温处理后的同种异体骨植入后宿主机体的细胞免疫学反应。组间对照的结果表明:经深低温冷冻处理后,确可降低异体骨的抗原性,而且异体骨抗原性的降低与深低温冷冻处理的时间有关。在建立的实验模型中,经冷冻处理一周后,异体骨的抗原性已降低与同基因骨移植组相似的程度。

Cellular immunological reaction of the subjects after transplantation of deep-frozen (at -196℃) al-logenic bone grafts was studied using the sensitive LDH - release assay of morden immunological technique. The antigenicity of allogenic bone was definitely decreased after deep-freezing treatment and related to the time of treatment. In the experimental models the antigenicity of the deep-frozen allogenic bone was reduced to the same level as that of isogenic bone when it had been frozen at-196℃ for 7 days.