HPLC测定人体内丙硫咪唑及其主要代谢物

THE DETERMINATION OF ALBENDAZOLE AND ITS MAIN METABOLITES IN HUMAN BY HPLC

本文报道了一种测定人体内丙硫咪唑及其砜和亚砜代谢物的高效液相色谱法。此法选用硅胶色谱柱,正已烷、氯仿、甲醇组成流动相,甲苯咪唑为内标物,检测波长292nm,在磷酸盐缓冲液环境中以醋酸乙酯为提取剂,同时测定包虫患者血清、囊液和胆汁中的药物及代谢产物。该法分离效果良好,回收率为85.4—117.2%,不同浓度水平除胆汁样品浓度为0.1μg/ml时重现性较差之外,其余均小于7.0%。血清、囊液、胆汁中各组分线性关系良好,相关系数均为0.999。经临床173个样品的测定表明,该法简单,结果满意。

This paper reports a method for determination of alben-dazole (ABZ) and its active metabolites-albendazole sulphone (ABZSN) and albendazole sulphoxide (ABZSX) in human serum, cystic liquid andbile by HPLC. It was chosen silica chromatography column and the mobilephase consisted of hexane, chloroform and methanol (80/13/7, v/v) at aflow rate 1.5ml/min. The wavelength of UV detector was 292nm. Mebend-azole was used as an internal standard and using phosphate buffered(pH7.4)ethylacetate as an extractant. Under the conditions described, the effect ofchromatographic seperation was satisfactory. Recoveries of ABZ, ABZSN,ABZSX in serum, cystic liquid and bile were 85.4-117. 2%. The precision(CY%) at different levels were all less than 7.0% except ABZSX in bilewas 23.64% at 0. 1μg/ml. The linearities of benzimidazoles in serum, cysticliquid and bile were obtained and correlation coefficients were all 0. 999.The clinical results of HPLC analysis proved that the method was simpleand reliable by mesurement for 173 samples of hydatid cystic patients.