早期心肌缺血的病理学诊断方法探讨肌酸激酶的免疫组化染色法

An Approach to the Pathologic Diagnosis of Early Myocardial Ischemia ──immuno-histochemical staining method for creatine kinase

对实验动物的缺血心肌进行ＰＡＰ法染色，结果心肌缺血４０ｍｉｎ后，着色即有变化，并随缺血时间的延长而逐渐明显，证实肌酸激酶的免疫组化染色是一种早期诊断心肌缺血的特异性好、敏感性高的重要辅助方法。

Pathologic diagnosis of early myocardial ischemia is a very important problem. We tried to research the specificity and sensitivity of creatine kinase immunohistochemical stain for the diagnosis of early myocardial ischemia. At first,the pure antigen was extracted from the skeletal muscles of SD rats. The rabbit was immunized and anti-rat antibody was obtained.The left coronary artery of female SD rat was ligated in order to make the myocardium ischemic 20, 40, 60, 120 and 140 min after ligation, the heart was taken out, the myocasdial specimens were embeded in paraffin and their sections were stained according to PAP method. The results showed that discolouration of the ischemic myocardium accurred if ischemic time was more than 40 min. So,we conclude that the creatine kinase PAP staining method is an effective diagnostic method and has higher specificity and sensitivity for early myocardial ischemia.