重组人肿瘤坏死因子对白血病细胞生长抑制的研究

The Suppressive Effect of Recombinant Human Tumor Necrosis Factor-αon Leukemic Cells

应用ＭＴＴ比色法研究了重组人肿瘤坏死因子（ｒｈＴＮＦα）对白血病细胞株ＨＬ６０和Ｋ５６２生长的抑制作用。实验结果表明，ｒｈＴＮＦα对白血病细胞生长的抑制作用有时相和剂量依赖关系。ｒｈＴＮＦα与ＨＬ６０和Ｋ５６２细胞分别培养１２ｈ，抑制率仅有６．２％和４．３％。当作用时间延长到２４ｈ时，抑制度分别达到５５．４％和４７．１％。ｒｈＴＮＦα的用量由０．０４μｇ／２×１０５细胞增加到５μｇ／２×１０５细胞时，对ＨＬ６０和Ｋ５６２细胞的生长抑制率也分别由１６．８％和６．４％增长到７７．１％和５９．３％。上述两种依赖关系的实验数据经单因素方差处理，有显著性差异。ｒｈＴＮＰα同抗肿瘤药Ｖｐ１６合用对白血病细胞生长的抑制有叠加作用。ｒｈＴＮＦα加入急性粒细胞白血病患者骨髓细胞培养体系后，对白血病细胞集落形成单位（ＣＦＵ－Ｌ）的生长有一定抑制作用。当ｒｈＴＮＦα为１μｇ／５×１０５白血病细胞时，对ＣＦＵ－Ｌ的抑制率可达４３．４％。

The effect of recombinant human tumor necrosis factor-a(rhTNFα)on leukemic cells was investigeted.The suppressive effect of rhTNF on HL60 and K562 leukemic cells was time and dose dependent.After incubation for 24 hours,the suppressive effect of rhTNFαon HL60 and K562 cells should be above 1μg at 2×105 leukemic cells.Our work indicated that rhTNFαwhen used conjointly with Vp16 exerted a synergetic action on inhibiting the growthof leukemic cells and that these results of two methods were generally the same. The suppressive effect of rhTNFαon colony forming unit-leukemia(CFU-L)in two patiensts with acute myelogenous leukemia was shown.These observations suggested that the rhTNFαcould be used to treat the patient with acute myelogenous leukemia.