蜂窝哈夫尼亚菌脂多糖核心区的免疫学特征

Immunological Characteriotics of Lipopolysaccharide Core Region of Hafnia Alvei

用单克隆抗体和多克隆抗血清对８株Ｈａｆｎｉａａｌｖｅｉ（Ｈ．ａｌｖｅｉ）的脂多糖核心区进行了测定，结果如下：①用ＫＤＯ－特异性单克隆抗体（克隆１４和２０）分别在６株和５株Ｈ．ａｌｖｅｉ的脂多糖中检测到α－连接的ＫＤＯ侧链，②Ｅ．ｃｏｌｉ核心型（Ｒ＿１～Ｒ＿４）特异性单克隆抗体和吸收后单一特异性抗血清均不能与Ｈ．ａｌｖｅｉ的脂多糖核心发生反应。③Ｅ．ｃｏｌｉ－Ｒｃ（化学型）核心特异性单克隆抗体（ＷＮ１２２２－５）能与所有（８株）测试的Ｈ．ａｌｖｅｉ的脂多糖发生反应。结果表明，Ｈ．ａｌｖｅｉ含有类似于Ｅ．ｃｏｌｉ－ＫＤＯ侧链和Ｒｃ（化学型）核心的抗原结构，但两者己糖区核心型（Ｒ＿１～Ｒ＿４）抗原不同。

onoclonal antibodies (Mabs) and polyclonal antisera (Pabs) were used for characterizing the LPS core of 8 H. alvei strains :①Two anti-KDO Mabs (clone 14,20) detected α-linked KDO side chain in several (6, 5) but not all of the LPS. ②Mabs and Pabs specific for the R_1～R_4 core of E. coli did not react to the LPS core of H. alvei. ③The Mab (WN1 222-5) specific for the Rc chemistry core of E. coli reacted strongly with all LPS of H. alvei tested. We conclude that the LPS of H. alvei contains both eiptopes (α-linked KDO side chain,Rc corel similar to those occurring in E. coli, but they are different in the core (R_1～R_4)types.